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Enzo Life Sciences Bio-16-dUTP (50nmol)
Supplier: Enzo Life Sciences ENZ42811
Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Bio-16-dUTP can be used to produce biotinylated DNA probes in a variety of labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The resulting biotin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. Alternative name: Biotin-16-2'-deoxyuridine-5'-triphosphate. Concentration: 1mM. Quantity: Sufficient for approximately 50 reactions, following the recommended protocol of ENZ-GEN111. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water.
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