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ACROBiosystems Recombinant Protein;Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag (Avitag™);HEK293;25UG;TIT,TIGIT,VSIG9,VSTM3,VSIG-9,VSTM-3
Supplier: ACROBiosystems TITH82F125UG
Recombinant Protein;Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag (Avitag™);25UG;Biotinylated Human TIGIT, Fc Tag, Avi Tag (TIT-H82F1) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 85-115 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, Fc Tag, Avi Tag (Cat. No. TIT-H82F1) with a linear range of 10-156 ng/mL (QC tested).;FACS assay shows Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag (Cat. No. TIT-H82F1) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 μg/ml (Routinely tested).
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