Dojindo Molecular Technologies Inc Biotin Labeling Kit-NH2(3 SAMPLES), is used for preparation of biotin-labeled IgG, only requires 1 hour to have high recovery of conjugates, applicable for 50-200 UG IgG/sample.

Supplier:  Dojindo Molecular Technologies Inc LK0310

Biotin Labeling Kit-NH2 is mainly used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA), NH2-reactive biotin, a component of this kit, has succinimidyl groups (NHS) that react with amino groups on proteins or other molecules. The labeling process is very simple, just add the NH2- reactive Biotin to IgG solution (50-200 UG protein/sample) and incubate at 37°C for 10 minutes. An average of 5 to 8 biotin molecules conjugate to each IgG molecule. The number of biotin molecules per protein can be determined by HABA assay. Excess biotin molecules can be removed by a filtration tube. The molecular weight of the protein to be labeled with this kit should be greater than 50,000. This kit contains all of the necessary reagents for labeling, including the storage buffer for conjugates. Dojindo has various protein labeling reagents. (https://www.dojindo.com/labeling-chemistry/)

Catalog No. NC9561110