Antibody;25UG;Mouse IgG1 / kappa;The cross-reactivity with other coronaviruses has not been tested yet.;Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (NUN-S47L8) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.;This protein . the protein migrates as 27 kDa and 55 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).;Detection SARS-CoV-2 Nucleocapsid Protein by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody (Cat. No. NUN-S46) at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein. And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody (Cat. No. NUN-S47L8) at 0.05 µg/mL. Detection was performed using high sensitivity HRP-conjugated streptavidi