Recombinant Protein;200UG;Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (CD7-H82A3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).;This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 42.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) with a linear range of 5-20 ng/mL (QC tested).;Immobilized Biotinylated Human CD47, Mouse IgG2a Fc,Avitag (Cat. No. CD7-H82A3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD47 Antibody, Human IgG4 with a linear range of 1-10 ng/mL (Routinely tested).