Recombinant Protein;200UG;Biotinylated Human CD16b (NA1), His,Avitag (SPR & BLI verified) (CDB-H82E4) is expressed from human HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # AAA35881.1).The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 24.6 kDa. The protein migrates as 38-48 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Biotinylated Human CD16b (NA1), His,Avitag (SPR & BLI verified) (Cat. No. CDB-H82E4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 8.32 μM as determined in a SPR assay (Biacore T200) (QC tested). ;Immobilized Biotinylated Human CD16b (NA1), His,Avitag (SPR & BLI verified) (Cat. No. CDB-H82E4) on SA Chip, can bind Herceptin with an affinity cons