Recombinant Protein;25UG;Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag™&Flag Tag (IL2-H8210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) & P29459-1 (IL12A)).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 38.3 kDa | 23.8 kDa. The protein migrates as 32-35 kDa and 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. IL2-H8210) with a linear range of 0.039-0.625 μg/mL (QC tested).;Immobilized Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. IL2-H8210) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate.