Recombinant Protein;200UG;Biotinylated Human IL-8, His,Avitag (IL8-H82H3) is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 12.0 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Biotinylated Human IL-8, His,Avitag (Cat. No. IL8-H82H3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).;Immobilized Monoclonal Anti-Human IL-8 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-8, His,Avitag (Cat. No. IL8-H82H3) with a linear range of 0.06-0.2 ng/mL (Routinely tested).