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ACROBiosystems Biotinylated Human Nectin-4 (244-349) Protein, His, AviTag (MALS verified)
Supplier: ACROBiosystems NE4H82ED
Biotinylated human Nectin-4 extracellular domain (amino acids 244-349) is produced in HEK293 cells with an N-terminal polyhistidine tag and an AviTag; the AviTag is enzymatically labeled with biotin. The reagent is provided lyophilized and is suitable for binding assays, receptor-ligand studies, and detection applications.
- Amino acid range: Phe 244-Ser 349.
- Expression system: Human 293 cells (HEK293).
- Tags: N-terminal polyhistidine tag and AviTag.
- Biotinylation: Enzymatic biotin labeling of the AviTag lysine residue.
- Purity: Greater than 95% as determined by SEC-MALS and SDS-PAGE.
- Molecular weight: Calculated 15.2 kDa; migrates ~18 kDa and 19-25 kDa on reducing SDS-PAGE due to glycosylation.
- Available sizes: 25 μg and 200 μg.
- Storage: Lyophilized, stable at -20°C to -70°C for 12 months; reconstituted product stable at -70°C for 3 months.
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