Recombinant Protein;25UG;Biotinylated Human NKG2C&CD94, His,Avitag (NC4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 94 - Leu 231 (NKG2C) & Asp 57 - Ile 179 (CD94) (Accession # P26717-1 (NKG2C) & Q13241-1 (CD94)).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 34.3 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Biotinylated Human NKG2C&CD94, His,Avitag (Cat. No. NC4-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE,TS-ME01-1 (QC tested).;HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE immobilized on CM5 Chip can bind Human NKG2C&CD94 Protein, His,Avitag™ (Cat. No. NC4-H82E3) with an affinity constant of 63.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).