Recombinant Protein;200UG;Biotinylated Human TIGIT, Mouse IgG2a Fc,Avitag (TIT-H82F3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).;This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 41.6 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, Mouse IgG2a Fc,Avitag (Cat. No. TIT-H82F3) with a linear range of 1-10 ng/mL (QC tested).;Immobilized Biotinylated Human TIGIT, Mouse IgG2a Fc,Avitag (Cat. No. TIT-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TIGIT Antibody with a linear range of 2-16 ng/mL (Routinely tested).