Recombinant Protein;200UG;Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-M58D2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P22646-1 (CD3E) & P11942-1 (CD3G)).;Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.8 kDa (CD3E) and 15.2 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 20-23 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized CD3E Monoclonal Antibody (145-2C11) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-M58D2) with a linear range of 5-78 ng/mL (QC tested).