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ACROBiosystems ACROBiosystems Biotinylated SARS-CoV-2 Spike RBD (K417N, L452R, T478K), His,Avitag™(MALS verified)
Supplier: ACROBiosystems SPDC82EG25UG
Recombinant Protein;25UG;Biotinylated SARS-CoV-2 Spike RBD (K417N, L452R, T478K), His,Avitag (SPD-C82Eg) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N, L452R, T478K)). The mutations (K417N, L452R, T478K) were identified in the SARS-CoV-2 variants which emerged in India (AY.1/AY.2/Delta Plus).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 28.3 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Biotinylated SARS-CoV-2 Spike RBD (K417N, L452R, T478K), His,Avitag (Cat. No. SPD-C82Eg) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).;Immobilized Biotinylated SARS-CoV-2 Spike RBD (K417N, L452R, T478K), His,Avitag (Cat. No. SPD-C82Eg) at 1 μg/mL (100 μL/well) on
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