Sigma Aldrich Fine Chemicals Biosciences BioTracker NucViewR 530 Re

Supplier:  Sigma Aldrich Fine Chemicals Biosciences SCT105

Caspases are a family of protease enzymes that play essential roles in programmed cell death termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.The BioTracker NucView 530 Red Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (fLICA) assays the BioTracker NucView 530 Red Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate which is initially non-fluorescent penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells caspase-3/7

Catalog No. 50-290-6607