AVIDITY LLC BirA Biotin-Protein Ligase Kit, EC6.3.4.15, 40 μg enzyme, 1 mg/mL concentration, 2 x 1.5 mL BiomixA (0.5 M Bicine buffer, pH 8.3), 2 x 1.5 mL BiomixB (100 mM ATP, 100 mM Mg(OAc)₂, 500 μM d-biotin)

Supplier:  AVIDITY LLC EC6.3.4.15

The Avidity LLC BirA Biotin-Protein Ligase Kit provides a highly efficient method for the covalent biotinylation of peptides and proteins. Utilizing the BirA biotin-protein ligase enzyme, the kit facilitates the specific labeling of target sequences such as the AviTag™ peptide, enhancing reaction rates and minimizing potential issues with protein stability. Ideal for applications like affinity chromatography and sensitive protein detection, this kit is essential for robust labeling in research applications.

• Product type: Biotin-protein ligase reaction kit
• Enzyme source: E. coli (wild-type)
• Enzyme purity: >99%
• Required cofactor: ATP
• Biotinylation type: Covalent
• Standard kit enzyme concentration: 1 mg/mL
• Standard kit enzyme amount: 40 μg
• Main application: Protein labeling and detection
• Key benefit: High efficiency and specificity

Catalog No. NC2683380