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Medchemexpress LLC BirA protein, E. coli (His-MBP) | >95.0% | 10 UG
Supplier: Medchemexpress LLC HYP7547510UG
Recombinant biotin ligase (BirA) derived from Escherichia coli, expressed in E. coli and supplied as an N-terminal 6xHis-MBP fusion protein. The product is lyophilized with stabilizers and intended for research use; biological activity is measured by pyrophosphate generation in the biotinylation reaction.
- Expression host: Escherichia coli.
- Tags: N-terminal 6xHis and MBP.
- Protein length: 321 amino acids, full length.
- Purity: >95% by reducing SDS-PAGE.
- Formulation: lyophilized from Tris/NaCl with glycerol, trehalose, mannitol, and Tween-80.
- Activity assay: biotinylation measured via pyrophosphate generation.
- Storage and handling: store at -20°C; reconstitute to ≥100 μg/mL in ddH2O recommended.
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