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AVIDITY LLC BIRA500-RT Biotin Protein Ligase, 2 vials of 20 μL each, 40 μg total, Lyophilized reaction kit, Covalent addition of d-biotin via ATP, >99% purity
Supplier: AVIDITY LLC BIRA500RT
The BIRA500-RT Biotin Protein Ligase is a lyophilized reaction kit designed for efficient and targeted biotinylation. This process is crucial in various applications such as affinity chromatography, protein immobilization, and detection with anti-biotin antibodies or avidin conjugates. Optimized for use with AviTag™ peptides, the kit enhances reaction rates, reduces enzyme use, and shortens incubation times, minimizing issues related to proteases and protein instability.
- Enzyme name: BirA biotin-protein ligase
- EC number: 6.3.4.15
- Form: Lyophilized reaction kit
- Source: E. coli wild-type
- Purity: >99%
- Molecular weight: 33,500 Daltons
- Activity: >8000 Units/μg of BirA
- Recommended substrate: AviTag™ peptide sequences
- Stored lyophilized: 1 year at 4°C
- Reconstituted storage: 2 weeks at 4°C, long term at -80°C
- Price: $250.00
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