Bio Rad Laboratories BiSpyCatcher2, Recombinant Protein, 1mg

Supplier:  Bio Rad Laboratories TZC002

BiSpyCatcher2 (H-BiSpyC2) is a 29 kDa homodimer of SpyCatcher2 (TZC001) bridged with a flexible linker. Each SpyCatcher2 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity. BiSpyCatcher2 contains a His6-tag at the N-terminus. To avoid deamidation of asparagine, a N105D mutation was introduced (Hentrich et al. 2020) for each of the two Catchers.The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2+/Mg2+ not needed) and in the presence of detergents. The reaction also occurs inside cells (in vivo). Since the speed of the coupling reaction is concentration dependent, it is recommended to use BiSpyCatcher2 undiluted.All the different formats of BiSpyCatcher2 are compatible with Bio-Rad's recombinant HuCAL® antibodies with a SpyTag2

Catalog No. NC3682185