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Zymo Research Corporation Bisulfite Converted Universal MethylatedHuman DNA Standard, 1 µg/50µl
Supplier: Zymo Research Corporation D5015
The supplied DNA has been methylated at all cytosine positions within the CG dinucleotide context using M.SssI methyltransferase. Following methylation, the DNA was treated with sodium bisulfite according to the protocol described in our EZ DNA Methylation-Direct™ kit. In this kit the methylated cytosines are unmodified by sodium bisulfite, whereas all non-methylated cytosines are converted to uracil, which are detected as thymine in subsequent PCR.The included primer set is designed to amplify a fragment of the human MLH1 mismatch repair gene (GenBank Accession# U83845, nucleotides 804 through 986) and can be used directly with the Bisulfite Converted Universal Methylated Human DNA Standard.
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