MilliporeSigma™ BLaER1 Human B-cell Precursor Leukemia Cell Line

Description

BLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.

• Induction of differentiation is a therapeutic approach for cancer
• Treatment of leukemias with differentiation-inducing factors in combination with pro-apoptotic compounds has been shown to be highly effective
• Ectopic expression of the transcription factor C/EBPα causes reprogramming of leukemia cells, resulting in expression of a normal cell phenotype
• The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPα fused with the estrogen receptor hormone binding domain (ER) and GFP
• A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages
• Cells retained their phenotype even when C/EBPα was inactivated, a characteristic of genuine cell reprogramming
• C/EBPα induction also impaired cell tumorigenicity after transplantation into immunodeficient mice
• BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells
• The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination

Specifications

• Content And Storage: Store in liquid nitrogen
• Format: Vial
• Quantity: ≥ 1 x 10⁶ cells/vial