Blasticidin S is a nucleoside antibiotic isolated fromStreptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts by inhibiting peptide bond formation by the ribosome. Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 2 to 20 µg/ml depending on the cell line.Blasticidin, also used in gene selection. Resistance to blasticidin is conferred by the blasticidin resistance gene from bacillus cereus (bsr). Bsr codes for blasticidin deaminase.Used for selection of pUNO I plasmids in E. coli and mammalian cell cultures.Appearance: SolidBoiling Point : 772.1 °C at 760 mmHg (Predicted)Melting Point: 218.0-225.0 °C