Invitrogen™ Mouse BLC/CXCL13 ELISA Kit

Catalog No.	Quantity
EMCXCL13X10	10 x 96 Tests

Catalog No. EMCXCL13X10 Supplier Invitrogen™ Supplier No. EMCXCL13X10

Description

ELISA

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt’s Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.

Specifications

• Accession Number: O55038
• Assay Range: 1.37 to 1000 pg/mL
• Assay Sensitivity: 2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 55985
• Gene Symbol: Cxcl13
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: CXCL13
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins