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Ubpbio BOC-LRR-AMC, 5MG
Supplier: Ubpbio G3100
Fluorescent Peptides/Protein Substrates – BOC-LRR-AMC (UBPBio catalog# G3100, G3101) – Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
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