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Bachem Boc-Val-Pro-Arg-AMC
Supplier: Bachem 4003460.0050
Boc-Val-Pro-Arg-AMC Hydrochloride, 65147-04-8, C31H45N7O7, Mr 627.74, 50mg. Boc-VPR-AMC, a specific, highly fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex. The thrombin assay has been used for the indirect determination of coagulation factor III. Trypsin, tryptase from rat mast cells, and two trypsin-like enzymes from sperm of the ascidian Halocynthia roretzi, acrosin and spermosin, also hydrolyze this substrate. Furthermore, Boc-VPR-AMC is cleaved by the Kex2 endoprotease (kexin, previously known as proteinase yscF), a membrane-bound, calcium-dependent serine protease from yeast a-cells. Kinetic data for Thrombin: kcat = 105, Km = 21 uM.
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