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Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format
Description
Requires
Mini Gel Tank (Mfr. No. A25977)
Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for Western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt Bis-Tris Plus gels to obtain optimal results for day-to-day protein separation needs.
Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES or MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.
• Optimized run conditions—separate proteins using constant voltage in approximately 20 minutes
• Better protein integrity—neutral pH sample preparation process preserves proteins
• Improved protein separation—offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than tris-glycine based gels
• Wedge-shaped wells—easier sample loading and up to two times higher sample volume than other 1.0mm-thick gels
Specifications
Specifications
| For Use With (Equipment) | Mini Gel Tank, XCell SureLock Mini-Cell |
| Gel Percentage | 10% |
| Gel Size | Mini |
| Gel Thickness | 1.0 mm |
| Gel Type | Bis-Tris Plus |
| Recommended Applications | Denaturing |
| Sample Loading Volume | up to 60 μL |
| Separation Range | 3.5 to 260 kDa |
| Separation Type | Denaturing |
| Wells | 10-well |
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Safety and Handling
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