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Bioworld Borate Buffer, 0.05M, pH 8.5, 500 mL
Supplier: Bioworld 402200481
Borate Buffer A reagent used in electrophoresis buffer and plant tissue culture media. Technical Information:ACS GradepKa (@ 20°C): 9.24Chloride <.001%Phosphate <0.001%Sulfate <0.01%Calcium <0.005%Heavy metals (as Pb) <0.001%Iron <0.001%PH Value: 8.5Protocol: Borate Buffer pH 7.4-9.2Borax (sodium tetraborate) 0.2M = 76.2 gm/ml Na2B407*120H20 (MW = 381.37)Boric acid 0.2M = 12.37 gm/1 H3BO3 (MW = 61.83)Add boric acid to borax solution until desired pH is reached. Dilute to desired molarity with ddH20.
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