Invitrogen™ Bovine IFN-alpha ELISA Kit

Catalog No.	Quantity
EB5RBX10	10 x 96 Tests

Catalog No. EB5RBX10 Supplier Invitrogen™ Supplier No. EB5RBX10

Description

ELISA

Bovine IFN-alpha quantitates bovine IFN-alpha in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant bovine IFN-alpha.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P07348
• Assay Range: 0.12-30 ng/mL
• Assay Sensitivity: 0.12 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Bovine
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: alpha-2a interferon; Gm12597; ifa; Ifa1; Ifa2; IFL; IFN; IFN alpha; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN1@; IFNA; IFNA@; IFNA1; IFNA13; Ifna15; Ifna2; IFNA2A; IFNA2B; IFNA2C; IFNA3; IFNA6; Ifnaa; IFNAalphaIFN-alpha-1/13; IFNalpha; IFN-alpha; IFN-alpha 1b; IFN-alpha1; IFN-alpha-1; IFN-alpha-1/13; IFN-alpha-2; IFN-alpha-6; IFN-alphaA; IFN-alphaD; IFN-gamma; IFNα; INFA2; interferon; interferon alpha; interferon alpha 1; Interferon alpha 13 (IFNA13); interferon alpha 15; interferon alpha 1b; interferon alpha 2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; interferon alpha family gene 1; interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); interferon alpha protein; interferon alpha-1; Interferon alpha1 (IFNA1); interferon alpha-1 precursor; interferon alpha-1/13; Interferon alpha-1/13 (IFN-alpha-1/13); Interferon alpha-2; Interferon alpha-A; Interferon alpha-D; Interferon alpha-D (IFN alphaD); interferon type A1/A2; interferon type A3; interferon, alpha 1; interferon, alpha 2; interferon-alpha; interferon-alpha 1; interferon-alpha1; interferon-alpha-6; leIF A; LeIF D; MGC138207; MGC138505; MGC138507; MuIFN-alpha-A; novel interferon alpha (Ifna) family member; OTTMUSG00000007655alpha-2a interferon; Gm12597; ifa; Ifa1; Ifa2; IFL; IFN; IFN alpha; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN1@; IFNA; IFNA@; IFNA1; IFNA13; Ifna15; Ifna2; IFNA2A; IFNA2B; IFNA2C; IFNA3; IFNA6; Ifnaa; IFNAalphaIFN-alpha-1/13; IFNalpha; IFN-alpha; IFN-alpha 1b; IFN-alpha1; IFN-alpha-1; IFN-alpha-1/13; IFN-alpha-2; IFN-alpha-6; IFN-alphaA; IFN-alphaD; IFN-gamma; IFNα; INFA2; interferon; interferon alpha; interferon alpha 1; Interferon alpha 13 (IFNA13); interferon alpha 15; interferon alpha 1b; interferon alpha 2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; interferon alpha family gene 1; interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); interferon alpha protein; interferon alpha-1; Interferon alpha1 (IFNA1); interferon alpha-1 precursor; interferon alpha-1/13; Interferon alpha-1/13 (IFN-alpha-1/13); Interferon alpha-2; Interferon alpha-A; Interferon alpha-D; Interferon alpha-D (IFN alphaD); interferon type A1/A2; interferon type A3; interferon, alpha 1; interferon, alpha 2; interferon-alpha; interferon-alpha 1; interferon-alpha1; interferon-alpha-6; leIF A; LeIF D; MGC138207; MGC138505; MGC138507; MuIFN-alpha-A; novel interferon alpha (Ifna) family member; OTTMUSG00000007655;
• Gene ID (Entrez): 281843
• Gene Symbol: IFNA1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IFN alpha
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min