Invitrogen™ Bovine Progesterone Rapid ELISA Kit

Catalog No.	Quantity
EELR001	96 Tests

Catalog No. EELR001 Supplier Invitrogen™ Supplier No. EELR001

Description

ELISA

The Bovine Progesterone (Pg) ELISA quantitates Pg in serum and plasma. Principle of the method The Bovine Pg solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. Bovine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. A substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The enzyme substrate reaction is terminated by the addition of stop solution and the color turns yellow. The intensity of this signal, measured spectrophotometrically at a wavelength of 450±2 nm, is directly proportional to the concentration of target present in the original specimen. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not...

Progesterone is the major progestational steroid, secreted primarily by the corpus luteum and the placenta. Progesterone binds to the progesterone receptor, resulting in dissociation of heat shock proteins, receptor phosphorylation, and transcription activation through direct or indirect interaction with transcription factors. Progesterone acts on the uterus, the mammary glands and the brain. It is required in embryo implantation, pregnancy maintenance, and the development of mammary tissue for milk production. Progesterone, converted from pregnenolone, also serves as an intermediate in the biosynthesis of gonadal steroid hormones and adrenal corticosteroids. Therapeutic progesterone is a synthetic form of the endogenous hormone progesterone. This agent exerts inhibitory effects on estrogens by decreasing the number of estrogen receptors and increasing its metabolism to inactive metabolites. [National Center for Biotechnology Information. PubChem Compound Database; CID=5994].

Specifications

• Assay Range: 78.13-5000 pg/mL
• Assay Sensitivity: 23.24 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Bovine
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 11-alpha-hydroxyprogesterone; Agolutin; C21H30O2; Crinone; Luteohormone; OHP; Pregn-4-ene-3; Progesterone-11a-hemisuccinate11-alpha-hydroxyprogesterone; Agolutin; C21H30O2; Crinone; Luteohormone; OHP; Pregn-4-ene-3; Progesterone-11a-hemisuccinate;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Progesterone
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min