Invitrogen™ Human BRAK/CXCL14 ELISA Kit

Catalog No.	Quantity
EHCXCL14X5	5 x 96 Tests
EHCXCL14	96 Tests
EHCXCL14X10	10 x 96 Tests

Catalog No. EHCXCL14X5 Supplier Invitrogen™ Supplier No. EHCXCL14X5

Description

ELISA

The Human BRAK/CXCL14 (BRAK) ELISA quantitates Hu BRAK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BRAK. Principle of the method The Human BRAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.

Specifications

• Accession Number: O95715
• Assay Range: 700-21000 pg/mL
• Assay Sensitivity: 700 pg/mL
• Calibration Range: 700 pg/mL to 21000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human CXCL14. Other species not determined.
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110031L23Rik; 1200006I23Rik; AI414372; B-cell and monocyte-activating chemokine; BMAC; bolekine; BRAK; breast and kidney; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 14; chemokine BRAK; CXC; CXC chemokine in breast and kidney; CXC motif chemokine; C-X-C motif chemokine 14; C-X-C motif chemokine ligand 14; CXCL; Cxcl14; hypothetical protein LOC511771; Kec; Kidney-expressed chemokine CXC; KS1; MGC10687; MIP-2 gamma; MIP2G; MIP-2g; MIP2gamma; musculus CXC chemokine MIP-2gamma; NJAC; PSEC0212; SCYB14; small inducible cytokine B14; small inducible cytokine subfamily B (Cys-X-Cys), member 14; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK); small-inducible cytokine B14; tumor-suppressing chemokine; UNQ240/PRO2731110031L23Rik; 1200006I23Rik; AI414372; B-cell and monocyte-activating chemokine; BMAC; bolekine; BRAK; breast and kidney; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 14; chemokine BRAK; CXC; CXC chemokine in breast and kidney; CXC motif chemokine; C-X-C motif chemokine 14; C-X-C motif chemokine ligand 14; CXCL; Cxcl14; hypothetical protein LOC511771; Kec; Kidney-expressed chemokine CXC; KS1; MGC10687; MIP-2 gamma; MIP2G; MIP-2g; MIP2gamma; musculus CXC chemokine MIP-2gamma; NJAC; PSEC0212; SCYB14; small inducible cytokine B14; small inducible cytokine subfamily B (Cys-X-Cys), member 14; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK); small-inducible cytokine B14; tumor-suppressing chemokine; UNQ240/PRO273;
• Gene ID (Entrez): 9547
• Gene Symbol: Cxcl14
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Chemokine BRAK, MIP-2G, Small-inducible cytokine B14
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: UNQ240/PRO273, BMAC, BRAK, KEC, KS1, MIP-2 g, MIP2G, NJAC, SCYB14
• Target: CXCL14
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min