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BIOSEARCH TECHNOLOGIES INC (Lucigen) Bst DNA Polymerase, Exonuclease Minus 50,000 U/mL, 10,000 UHigh specific activity for DNA amplification and sequencing.
Supplier: BIOSEARCH TECHNOLOGIES INC 300281
Bst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5-3 polymerase activity and strand displacement activity, but it lacks 3-5 exonuclease activity. It also has reverse transcription activity. Bst DNA Polymerase, Exonuclease Minus, has higher strand displacement activity than that of other suppliers. The enzyme can be used in nucleic acid amplification methods* such as isothermal amplification, whole genome amplification (WGA), and multiple displacement amplification (MDA). It also can be used in Next Generation sequencing. This enzyme has optimal activity at 65C. It is suitable for sequencing DNA with high GC content and secondary structures. It is available in concentrations of 8,000 U/ml or 50,000 U/ml.
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