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ATCC BT-474 Clone 5; Breast Ductal Carcinoma; Human (Homo sapiens)
Supplier: ATCC CRL3247
BT-474 Clone 5 is an epithelial-like cell line clone that was originally isolated from the mammary gland of a 60-year-old, White female patient with ductal carcinoma. BT-474 (ATCC HTB-20) cells were grown in 10ug/mL Herceptin for several weeks until the resulting cells (clones) were defined as resistant to Herceptin treatment. These resistant cells were grown in standard RPMI with 10% fetal bovine serum for many years under different conditions but the cells enclosed have not been exposed to Herceptin after they were first treated. They were not grown with Herceptin to make sure they stayed resistant. However, these cells have remained resistant for 30+ passages and they still retain the overexpression of HER-2 and are resistant to growth inhibition due to Herceptin.
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