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IBL America Coxiella Burnetii Phase I IgG ELISA
Supplier: IBL America IB05036
Enzyme immunoassay (ELISA) for the determination of human IgG antibodies directed against Coxiella burnetii in phase 1 in serum or plasma (EDTA, citrate, heprin). For research use only, not for use in diagnostic procedures.The reaction is based on the specific interaction of antibodies with their corresponding antigen.The test strips of the microtiter plate are coated with specific antigens of the pathogen ofinterest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically.
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