Invitrogen™ Human C-Peptide ELISA Kit

Catalog No.	Quantity
BMS21912	96 Tests

Catalog No. BMS21912 Supplier Invitrogen™ Supplier No. BMS21912

Description

ELISA

The Human c-Peptide ELISA quantitates Hu c-Peptide in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu c-Peptide. Principle of the method The Human c-Peptide solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.

Specifications

• Accession Number: P01308
• Assay Range: 23-1500 pg/mL
• Assay Sensitivity: 2.9 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Sample Type: Plasma, Serum, Supernatant, Urine
• Substrate Type: Adaptor Proteins
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Cpeptide; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2-rs1; insulin; insulin 1; Insulin A chain; Insulin B chain; insulin I; insulin-1; Insulin-1 A chain; Insulin-1 B chain; IRDN; MODY10; preproinsulin; proinsulinCpeptide; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2-rs1; insulin; insulin 1; Insulin A chain; Insulin B chain; insulin I; insulin-1; Insulin-1 A chain; Insulin-1 B chain; IRDN; MODY10; preproinsulin; proinsulin; IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10
• Gene ID (Entrez): 3630
• Gene Symbol: INS
• Interassay CV: <10%
• Intraassay CV: <6%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Connecting Peptide, Insulin, Insulin A chain, Insulin B chain, preproinsulin, proinsulin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: C-Peptide
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr