Invitrogen™ Human C-Peptide ELISA Kit

Description

ELISA

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.

Specifications

• Accession Number: P01308
• Assay Range: 23 to 1500 pg/mL
• Assay Sensitivity: 2.9 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant, Urine
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3630
• Gene Symbol: INS
• Interassay CV: <10%
• Intraassay CV: <6
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
• Storage Requirements: 2°C to 8°C
• Target: C-Peptide
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs