Calcium phosphate transfection is a commonly used method for introducing exogenous DNA into eukaryotic cells DNA-calcium phosphate precipitate adheres to cells by interacting with their cell surface The exogenous DNA is subsequently internalized via cellular endocytosis ultimately delivering the DNA into target cells This method has been successfully applied to various cell lines to achieve transient or stable transfections HEK293 cells are most suitable for calcium phosphate transfection Hela and CHO cells can also be transfected using the calcium phosphate method with slightly lower transfection efficiency This product significantly enhancing transfection efficiency and reducing cytotoxicity Additionally the kit provides highly consistent pH values and salt concentrations ensuring reliable transfection efficiency across different experiments and meet for the transfection needs of most adherent and suspension cells