MilliporeSigma™ Calbiochem™ Argonaute-2 Inhibitor, BCI-137

Description

A cell-permeable, non-toxic dioxotetrahydroquinoxaline compound that mimics uridine and directly and reversibly interacts with the microRNA (miRNA) binding domain of Argonaute-2 (Ago2; K_D = 126μM) and significantly inhibits binding of miR-20a, miR-26a, miR-107, miR-223, and let-7a to A

A cell-permeable, non-toxic dioxotetrahydroquinoxaline compound that mimics uridine and directly and reversibly interacts with the microRNA (miRNA) binding domain of Argonaute-2 (Ago2; K_D = 126μM) and significantly inhibits binding of miR-20a, miR-26a, miR-107, miR-223, and let-7a to Ago2. However, it does not affect Ago2 stability in cells. Displaces Ago2 from the miR-155 host gene promoter region (70% reduction in Ago2 association) and increases histone H4 acetylation (∽300%) in NB4 leukemia cell line. Promotes retinoic acid-induced differentiation of NB4 cells to granulocytes.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Specifications

• Color: Gray-white
• Description: 97% by HPLC
• Target Proteins: Argonaute-2
• Molecular Formula: C₁₁H₁₁N₃O₆S
• Solubility: DMSO
• Content And Storage: Protect from light
• Form: Solid
• Quantity: 25 mg