MilliporeSigma™ Calbiochem™ Deoxyhypusine Synthase Inhibitor, GC7

The Deoxyhypusine Synthase Inhibitor, GC7, also referenced under CAS 150333-69-0, controls the biological activity of Deoxyhypusine Synthase.

Manufacturer:  MilliporeSigma™ 25954510MG

Catalog No. 25-954-510MG

Description

A cell-permeable inhibitor that targets the spermidine-binding site of Deoxyhypusine Synthase (K_i = 9.7 nM), an enzyme which is responsible for the first step hypunisation of eukaryotic initiation factor 5A (eIF5A). In vivo, it demonstrates almost complete cessation of hypusine formation in CHO cells at ≥ 1µM. Several studies indicate this compound as a useful tool for the down-regulation of eIF5A, a protein essential for cell proliferation. For example, it exhibits an anti-proliferative effect on both BCR-ABL -positive and -negative leukemia cell lines (K562 and HL-60) dose-dependently, at concentrations between 5µM and 40µM. In addition, it is shown to significantly impair the growth and invasion of primary HCC cells (HepG2 and Hep3B) in the 10-20µM range, and against non-tumorigenic CL-48 cells at concentrations as high as 40µM, with no apparent cytotoxicity. At 25µM, GC7 treatment is shown to reverse eIF5a-mediated Skeletal Muscle Stem Cell Differentiation.

Specifications

• Description: >95% by NMR
• Storage Requirements: Hygroscopic
• CAS: 150333-69-0
• Formula Weight: 270.35g/mol

• Format: White powder
• Quantity: 10mg
• Molecular Formula: C₈H₂₂N₄O₄S
• Solubility: H₂O