MilliporeSigma™ Calbiochem™ InSolution™ Foxo1 Inhibitor, AS1842856

Catalog No.	Quantity
50-608-10001	5 mg

Catalog No. 50-608-10001 Supplier MilliporeSigma™ Supplier No. 5060810001

Description

A cell-permeable oxodihydroquinoline

A cell-permeable oxodihydroquinoline that preferentially inhibits the transcription activity of Forkhead box O family member Foxo1 over that of the functionally related Foxo3a and Foxo4 (70%, 20%, and 3% inhibition, respectively, in HepG2-based reporter assays; [AS184256] = 100nM) in a dose-dependent manner (IC₅₀ = 33nM) via direct binding of the non-Ser256-phosphorylated/active Foxo1, but not the Ser256-phosphorylated/inactive form of Foxo1. Shown to inhibit gluconeogenesis in rat heptoma Fao cultures (IC₅₀ = 37 and 130nM against PEPCK and G6Pase mRNA level; IC₅₀ = 43nM against glucose production) in vitro and in murine liver of both normoglycemic ICR mice (60% and 45% inhibition of hepatic G6Pase and PEPCK mRNA level, respectively; three 100mg/kg p.o. dosages at 0, 12, 24 h) and diabetic db/db mice (86%, 70%, 56%, 48%, 35%, and 31% inhibition of hepatic G6Pase, ABCG8, ABCG5, PEPCK, IL-1β, and apoCIII mRNA level, respectively; three 100mg/kg p.o. dosages at 0, 12, 24 h) during a 26-hour fasting period in vivo .

Specifications

• Formulation: A 50 mM (5mg/288μL) sterile-filtered solution of Foxo1 Inhibitor, AS1842856 in DMSO.
• Description: 98% by HPLC
• Target Proteins: Foxo1
• Molecular Formula: C₁₈H₂₂FN₃O₃
• Formula Weight: 347.4g/mol
• Content And Storage: Avoid Freeze-Thaw Cycles, Hygroscopic, Protect from Light
• Form: Liquid
• Quantity: 5 mg