MilliporeSigma™ Calbiochem™ MRN-ATM Pathway Inhibitor, Mirin

Description

The MRN-ATM Pathway Inhibitor, Mirin, also referenced under CAS 299953-00-7, controls the biological activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex. This small molecule/inhibitor is primarily used for Cell Structure applications.

A cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC₅₀ = 12μM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (∽1.6-fold at 50μM) and dramatically increase mitotic cell polulation (∽10-fold at 25μM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.

Specifications

• Color: Reddish-orange
• Description: 95% by HPLC
• CAS: 299953-00-7
• Molecular Formula: C₁₀H₈N₂O₂S
• Formula Weight: 220.3g/mol
• Solubility: DMSO or EtOH
• Content And Storage: Protect from light
• Form: Powder
• Quantity: 10 mg