MilliporeSigma™ Calbiochem™ NBD-Binding Peptide II, Cell Permeable, CTP-NBD

A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFα-stimulated NF-κB activity (IC₅₀∽ 50μM in HCT116 cells).

Supplier:  MilliporeSigma™ 4800265MG

Catalog No. 48-002-65MG

Description

A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFα-stimulated NF-κB activity (IC₅₀ ∽ 50μM in HCT116 cells). Acts by interfering with the formation of active IKK complex and thereby prevents IκBα phosphorylation and its subsequent proteasomal degradation. Also blocks LPS-mediated increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activities. Colon-targeted capsules containing CTP-NBD are shown to reduce 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rodent models (4.5mg/kg/,p.o.) in vivo and diminish activities of myeloperoxidase and several inflammatory mediators. Antennapedia fused NBD cell-permeable peptide is also available ( Cat. No. 480025).

Specifications

• Color: White
• Description: 97% by HPLC
• Formula Weight: 2846.2g/mol
• Content And Storage: Hygroscopic, Protect from light
• Quantity: 5 mg

• Formulation: Supplied as a trifluoroacetate salt.
• Target Proteins: NEMO-binding peptide
• Solubility: H₂O
• Form: Solid