MilliporeSigma™ Calbiochem™ RAD51-Stimulatory Compound-1, RS-1

Description

A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis.

A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 and TALEN-mediated knock-in efficiencies.

Specifications

• Color: Light Beige
• Description: 95% by HPLC
• CAS: 312756-74-4
• Molecular Formula: C₂₀H₁₆Br₂N₂O₃S
• Formula Weight: 524.2g/mol
• Solubility: DMSO or EtOH
• Content And Storage: Protect from light
• Form: Solid
• Quantity: 10 mg