MilliporeSigma™ Calbiochem™ Rho Inhibitor II, Y16

A cell-permeable pyrazolidinedione compound that is shown to target RhoGEF DH-PH domain junction with high affinity (K_d = 65nM) and effetively prevent RhoGEFs LARG, p115, and PDZ from interacting with RhoA, while displaying little potency against DBL-RhoA, LBC-RhoA, intersectin-Cdc42, or TrioN-Cdc42 interact

Supplier:  MilliporeSigma™ 5.04043.0001

Catalog No. 50-404-30001

Description

A cell-permeable pyrazolidinedione compound that is shown to target RhoGEF DH-PH domain junction with high affinity (K_d = 65nM) and effetively prevent RhoGEFs LARG, p115, and PDZ from interacting with RhoA, while displaying little potency against DBL-RhoA, LBC-RhoA, intersectin-Cdc42, or TrioN-Cdc42 interaction. Shown to completely prevent serum-induced activation of cellular RhoA, but not Cdc42 or Rac1, in NIH-3T3 cultures (10μM) and RhoA downstream signaling events. Greatly synergizes with Rho GEF-binding domain blocker Rhosin (Cat. No. 555460) in blocking RhoA-LARG interaction and in preventing cellular RhoA activation (both drug at 5μM).

Specifications

• Color: Orange
• Target Proteins: RhoGEF
• Formula Weight: 384.4g/mol
• Content And Storage: Protect from light
• Quantity: 10 mg

• Description: 95% by HPLC
• Molecular Formula: C₂₄H₂₀N₂O₃
• Solubility: DMSO
• Form: Powder