Invitrogen™ Canine IL-12 p40 ELISA Kit

Catalog No.	Quantity
EC10RBX5	5 x 96 Tests
EC10RB	96 Tests

Catalog No. EC10RBX5 Supplier Invitrogen™ Supplier No. EC10RBX5

Description

ELISA

Canine IL-12 p40 quantitates canine IL-12 p40 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant canine IL-12 p40.

IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Specifications

• Accession Number: CLMF; CLMF p35; CLMF p40; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; Il12; IL-12 p40 subunit; IL-12 subunit p35; IL-12 subunit p40; IL12, subunit p40; IL-12.p40; IL12A; IL-12 A; Il12b; IL-12 B; Il12p40; Il-12p40; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 p40 subunit; interleukin 12, p40; interleukin 12 B; interleukin 12 B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); Interleukin12; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 chain; interleukin-12 p40 subunit; interleukin-12 subunit beta; interleukin-12p40; interleukin-23 p40 subunit; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p40; RP23-388G23.1
• Assay Range: 24.58 to 6,000 pg/mL
• Assay Sensitivity: 24 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 403976
• Gene Symbol: Il12b
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-12 p40
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins