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Thermo Scientific™ CaptureSelect™ AAT-XL RoboColumn™ Pre-packed columns

Catalog No. 5943832200
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Quantity:
1 Strip (8 Pieces), 200 μL

Description

Thermo Scientific™ CaptureSelect™ RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

The CaptureSelect AAT-XL Affinity Matrix in this RoboColumn has been specifically designed for the purification of native human alpha-1 antitrypsin (AAT) from complex sources like plasma/serum and recombinant human AAT from cell culture supernatants. The high selectivity and yield obtained using CaptureSelect AAT-XL Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in a single step.

Features of this affinity matrix include:

  • Highly selective for native and recombinant human AAT
  • High recovery and yield obtained in a single purification step
  • Mild elution at pH 6
  • Excellent scalability
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

  • Column size: 200 μL, 1 strip, 8 columns (8-row)
  • Column dimension: 5-mm diameter, 10-mm bed height
  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect AAT-XL affinity ligand
  • Ligand coupling: epoxide coupling
  • Binding capacity: 15 mg AAT/mL resin depending on flow rate, column height, and contact time
  • Elution conditions: 20 mM Tris, 2.0 M MgCl2, pH 6.0
  • Formulation buffer: 20% (v/v) ethanol
  • Operating pressure: 2 bar (maximum)
  • Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.

Specifications

Capacity (Metric) 200 μL
Particle Size 65 ±10 μm
Product Line CaptureSelect™
Quantity 1 Strip (8 Pieces), 200 μL
Format Robo Column
Column Type Affinity, Prepacked
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