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  6. Thermo Scientific™ CaptureSelect™ Biotin Anti-Free LC-kappa (Human) Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-Free LC-kappa (Human) Conjugate

Catalog No. 7103292500
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Quantity:
100 μg
500 μg

Description

Thermo Scientific™ CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) that binds highly selectively to free human kappa light chains and dimers thereof.

CaptureSelect Biotin Anti-Free LC-kappa (Hu) Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) that binds highly selectively to free human kappa light chains and dimers thereof. The functionality is based on recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect Biotin Anti-Free LC-kappa (Hu) Conjugate allows you to:

  • Detect, quantitate, and characterize free human kappa light chains in (crude) antibody preps
  • Avoid cross-binding with human kappa light chains present in intact and properly folded antibodies and Fab fragments of any human antibody isotype such as IgG, IgA, IgM, IgD, and IgE

Applications for CaptureSelect Biotin Anti-Free LC-kappa (Hu) Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Specifications

Content And Storage Store in freezer (-5°C to -30°C).
Form Liquid
Product Line CaptureSelect™
Quantity 500 μg
Type Conjugate
Label or Dye Biotin & Other Haptens
Ligand Type Antibody
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