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Thermo Scientific™ CaptureSelect™ Factor X Affinity Matrix

Catalog No. 2943702005
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Quantity:
5 mL
10 mL
50 mL
250 mL
1 L

Thermo Scientific™ CaptureSelect™ Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.

CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.

Features of the CaptureSelect Factor X Affinity Matrix include:

  • Highly selective for human Factor X with no affinity for other human plasma proteins
  • Binds both Factor X and Factor Xa in the presence of calcium
  • Enables efficient purification of Factor X with high yields and retained activity
  • Mild elution at neutral pH using EDTA
  • Offers excellent scalability
  • Non-animal-derived

Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: agarose-based
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect Factor X affinity ligand
  • Ligand coupling method: epoxide
  • Binding capacity: >10 mg/mL resin
  • Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7
  • Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity >10 mg/mL
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Matrix Agarose-based
Content And Storage Store in refrigerator (2°C to 8°C).
Shipping Condition Wet Ice
Particle Size 65 ±10 μm
Form Agarose Bead
Product Line CaptureSelect™
Quantity 5 mL
Target Protein
Type Affinity Matrix
Ligand Type Factor X affinity ligand
Stationary Phase Factor X
Column Type Agarose Resin
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