Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. 5943212005

Thermo Scientific™ CaptureSelect™ KappaXP MiniChrom™ Pre-packed columns

Catalog No. 5943212005
Click to view available options
Quantity:
5 mL

Description

The Thermo Scientific™ CaptureSelect™ KappaXP MiniChrom™ column is designed for bench-scale resin screening, process development, and sample preparation.

CaptureSelect KappaXP MiniChrom™ column is designed for bench-scale resin screening, process development, and sample preparation.

This pre-packed column is ready to connect and has the following features:

  • Volume of 5 mL (0.8 x 10 cm)
  • Direct connection to standard chromatography systems
  • Packed with CaptureSelect KappaXP Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.

CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams.

Features of this affinity matrix include:

  • Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
  • Mild elution for Fab fragments and antibodies
  • Human-specific, no binding to bovine antibodies
  • Excellent scalability
  • Excellent pH stability up to 100 mM NaOH (limited exposure)
  • Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect KappaXP affinity ligand
  • Ligand coupling method: epoxide coupling
  • Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
  • IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height
  • Elution conditions: 20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2, pH 6
  • Column dimensions: 5 mL (0.8 x 10 cm)
  • Flow characteristics: 75–150 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Bed Volume 5 mL
Flow Rate 75 to 150 cm/hr. (Up to 2 bar)
Content And Storage Store in refrigerator (2°C to 8°C).
Diameter (Metric) 0.8 cm
Length (Metric) 10 cm
Shipping Condition Wet Ice
Particle Size 65 ±10 μm
Product Line CaptureSelect™
Quantity 5 mL
Type Pre-packed Column
Ligand Type KappaXP affinity ligand
Stationary Phase KappaXP Affinity Matrix with KappaXP Ligand
Format Chromatography Cartridge
Column Type MiniChrom
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.