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Thermo Scientific™ CaptureSelect™ KappaXP RoboColumn™ Pre-packed columns

Catalog No. 5943212200
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59-432-12200 200 μL
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Description

Thermo Scientific™ CaptureSelect™ RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect KappaXP matrix recognizes the kappa light-chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams.

Features of this affinity matrix include

  • Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
  • Mild elution for Fab fragments and antibodies
  • Human-specific, no binding to bovine antibodies
  • Excellent scalability
  • Excellent pH stability up to 100 mM NaOH (limited exposure)
  • Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

  • Column size: 200 μL, 1 strip, 8 columns (8-row)
  • Column dimension: 5-mm diameter, 10-mm bed height
  • Matrix: agarose based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect KappaXP affinity ligand
  • Ligand coupling method: epoxide coupling
  • Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
  • IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height, and residence time
  • Elution conditions: 20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2, pH6
  • Formulation buffer: 20% (v/v) ethanol
  • Max operating pressure: 2 bar
  • Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.

Specifications

Content And Storage Store in refrigerator (2–8°C).
Shipping Condition Wet Ice
Particle Size 65 ±10 μm
Product Line CaptureSelect
Quantity 200 μL
Type Column
Stationary Phase KappaXP Affinity Matrix with KappaXP Ligand
Format 8-well Strips

For Research Use Only. Not for use in diagnostic procedures.

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