This kit detects cTn-I in canine serum plasma and tissue homogenates using a sandwich ELISA technique It offers a quantitative detection range of 23 5 pg/mL to 1500 pg/mL with a sensitivity of 5 8 pg/mL enabling precise measurement of low-abundance targets The assay requires 50-100ul of sample per test and features an optimized protocol with an incubation time of 1-5 hours for flexibility in experimental workflows Detection is performed at 450 nm wavelength ensuring reliable optical readouts Designed for researchers studying cardiac biomarkers this kit provides standardized components for consistent performance in veterinary diagnostics or cardiovascular research applications while maintaining rigorous quality controls throughout the analytical process