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STEMCELL Technologies Caspase-3/7 Activity Flow Cytometer Kit, Green, 100 Tests
Supplier: STEMCELL Technologies 1000923
Efficiently detect active caspase-3 in mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Flow Cytometry Kit, Green. This reagent kit uses a quick and simple staining protocol that is highly sensitive and compatible with standard flow cytometers.Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit includes TF2-DEVD-FMK—a cell-permeable and non-toxic fluorescent substrate—as well as an assay buffer and propidium iodide. When added to apoptotic cells, TF2-DEVD-FMK irreversibly binds to activated caspase-3, resulting in the retention of the green fluorescent reagent within cells that can be detected by flow cytometry.
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